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rabbit anti fap  (Novus Biologicals)


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    Structured Review

    Novus Biologicals rabbit anti fap
    Rabbit Anti Fap, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+fap/Fibroblast+Activation+Protein+alpha%2FFAP+Antibody+(JA56-11)/pm41776828-79-91-92
    Average 94 stars, based on 4 article reviews
    rabbit anti fap - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Staining:

    Article Title: Exposure of endothelial cells to Doxorubicin inhibits Extracellular matrix production by dermal fibroblasts in a paracrine manner.
    Article Snippet: .. 2 For immunofluorescent staining, cells were fixed after treatment with 4% paraformaldehyde 3 for 10 minutes at RT and subsequently permeabilized using 0.1% (v/v) Triton X-100 in PBS 4 for 10 min. After extensive PBS washes, cells were blocked with a solution of 1% BSA and 5 0.1% NaN3 in PBS and then incubated with primary antibodies: mouse-anti-α-SMA (Dako; 6 M0851, 1:1000) or rabbit-anti-FAP (NOVUS; NBP2-66844, 1:1000) for 2 hours at RT. .. After 7 washing three times with PBS, cells were incubated with secondary antibodies: goat-anti-rabbit 8 IgG(H+L) A633 (Thermo Fisher Scientific; A21070, 1:500 dilution) for FAP or goat-anti- 9 mouse IgG(H+L) A488 (Thermo Fisher Scientific; A11029,1:500 dilution) for α-SMA, for 1 10 hour at RT.

    Article Title: Exposure of endothelial cells to doxorubicin inhibits extracellular matrix production by dermal fibroblasts in a paracrine manner
    Article Snippet: .. For immunofluorescent staining, cells were fixed after treatment with 4% paraformaldehyde for 10 min at RT and subsequently permeabilized using 0.1% (v/v) Triton X-100 in PBS for 10 min. After extensive PBS washes, cells were blocked with a solution of 1% BSA and 0.1% NaN 3 in PBS and then incubated with primary antibodies: mouse-anti-α-SMA (Dako; M0851, 1:1000) or rabbit-anti-FAP (NOVUS; NBP2-66844, 1:1000) for 2 h at RT. .. After washing 3 times with PBS, cells were incubated with secondary antibodies: goat-anti-rabbit IgG(H + L) A633 (Thermo Fisher Scientific; A21070, 1:500 dilution) for FAP or goat-anti-mouse IgG(H + L) A488 (Thermo Fisher Scientific; A11029, 1:500 dilution) for α-SMA, for 1 h at RT.

    Incubation:

    Article Title: Exposure of endothelial cells to Doxorubicin inhibits Extracellular matrix production by dermal fibroblasts in a paracrine manner.
    Article Snippet: .. 2 For immunofluorescent staining, cells were fixed after treatment with 4% paraformaldehyde 3 for 10 minutes at RT and subsequently permeabilized using 0.1% (v/v) Triton X-100 in PBS 4 for 10 min. After extensive PBS washes, cells were blocked with a solution of 1% BSA and 5 0.1% NaN3 in PBS and then incubated with primary antibodies: mouse-anti-α-SMA (Dako; 6 M0851, 1:1000) or rabbit-anti-FAP (NOVUS; NBP2-66844, 1:1000) for 2 hours at RT. .. After 7 washing three times with PBS, cells were incubated with secondary antibodies: goat-anti-rabbit 8 IgG(H+L) A633 (Thermo Fisher Scientific; A21070, 1:500 dilution) for FAP or goat-anti- 9 mouse IgG(H+L) A488 (Thermo Fisher Scientific; A11029,1:500 dilution) for α-SMA, for 1 10 hour at RT.

    Article Title: Exposure of endothelial cells to doxorubicin inhibits extracellular matrix production by dermal fibroblasts in a paracrine manner
    Article Snippet: .. For immunofluorescent staining, cells were fixed after treatment with 4% paraformaldehyde for 10 min at RT and subsequently permeabilized using 0.1% (v/v) Triton X-100 in PBS for 10 min. After extensive PBS washes, cells were blocked with a solution of 1% BSA and 0.1% NaN 3 in PBS and then incubated with primary antibodies: mouse-anti-α-SMA (Dako; M0851, 1:1000) or rabbit-anti-FAP (NOVUS; NBP2-66844, 1:1000) for 2 h at RT. .. After washing 3 times with PBS, cells were incubated with secondary antibodies: goat-anti-rabbit IgG(H + L) A633 (Thermo Fisher Scientific; A21070, 1:500 dilution) for FAP or goat-anti-mouse IgG(H + L) A488 (Thermo Fisher Scientific; A11029, 1:500 dilution) for α-SMA, for 1 h at RT.

    Article Title: Exposure of endothelial cells to doxorubicin inhibits extracellular matrix production by dermal fibroblasts in a paracrine manner
    Article Snippet: Samples were separated using NuPAGE Bis-Tris Mini Protein Gels (4% to 12%, 1.0 to 1.5 mm, NP0322BOX, Thermo Scientific, USA) at 80 V for 20 min, followed by 120 V for 45 min. Proteins were transferred from gel onto a PVDF membrane at 120 V for 1 h. After extensive washing with TBST, membranes were blocked with 5% (w/v) skim milk in TBST for 1 h at RT. .. Membranes were incubated overnight at 4 °C with primary antibodies: mouse-anti-α-SMA (Dako; M0851, 1:1000), rabbit-anti-FAP (NOVUS; NBP2-66844, 1:1000), rabbit-anti-Collagen I (Abnova; PAB10190 , 1:1000 dilution), or rabbit-anti-S100A4 (abcam; ab197896, 1:1000). .. Following 3 washes with TBST [TBS with 0.1%(v/v) tween 20], the membranes were incubated with secondary antibodies: rabbit-anti-mouse IgG-HRP (Dako; P0260, 1:2500 dilution) for α-SMA or goat-anti-rabbit IgG-HRP (Dako; P0448, 1:2500 dilution) for FAP, Collagen I and S100A4, for 1 h at RT.

    Article Title: Exposure of endothelial cells to Doxorubicin inhibits Extracellular matrix production by dermal fibroblasts in a paracrine manner.
    Article Snippet: .. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 D ow nloaded from https://academ ic.oup.com /toxsci/advance-article/doi/10.1093/toxsci/kfag027/8504434 by guest on 09 M arch 2026 9 1 Membranes were incubated overnight at 4 °C with primary antibodies: mouse-anti-α-SMA 2 (Dako; M0851, 1:1000), rabbit-anti-FAP (NOVUS; NBP2-66844, 1:1000), rabbit-anti3 Collagen I (Abnova; PAB10190, 1:1000 dilution), or rabbit-anti-S100A4 (abcam; ab197896, 4 1:1000). .. Following three washes with TBST (TBS with 0.1%(v/v) tween 20), the membranes 5 were incubated with secondary antibodies: rabbit-anti-mouse IgG-HRP (Dako; P0260, 1:2500 6 dilution) for α-SMA or goat-anti-rabbit IgG-HRP (Dako; P0448, 1:2500 dilution) for FAP, 7 Collagen I and S100A4, for 1 hour at RT.



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